[PDF] Using of Hibiscus Sabdariffa extract as a natural histological stain of the Skin | Semantic Scholar (2024)

Hibiscus Sabdariffa solution can be used effectively in staining of appendicular tissues instead of H&E staining method, and shows the best staining quality within 60 minutes time duration.

The results obtained indicate that Hibiscus solution has the potential for use in diagnostic bacteriology in formalin-fixed paraffin-embedded tissue section.

The results obtained indicate that hibiscus plant extract have the potential for use in the morphological identification of fungi in formalin-fixed paraffin-embedded tissue section.

Camwood (Baphia nitida) alcoholic extract is a suitable counter stain for haematoxylin stains especially on liver and kidney tissues and may be a potential alternative to eosin in the demonstration of tissue morphology.

Anthocyanins could potentially be used as viable histological stains having advantages of availability, feasibility, color stability and nontoxicity, although numerous modifications to improve longevity of staining solution and staining characteristics are warranted by further research.

The result showed that the most contrast prepared slide was used red dragon fruit extract in 60% concentration, which use to arrange practical guide in observation of plant tissues which is validated by material expert.

Two extracts from the petals of common mallow were used to create both a blue nuclear stain and a red counterstain, which is remarkably similar to that of hemalum plus eosin.

The dye extracted from the corolla tube of Nyctanthes arbor‐tristis is a potential natural dye and could be used as biological stain for staining plant tissues.

This literature review explores the natural products and their efficiency to be used as alternatives for chemicals in the histopathology lab.

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Hibiscus Sabdariffa is an efficient natural cheap substitute of eosin in the ordinary stain H&E (Hematoxylin and Eosin) for histological sections.

Hibiscus Sabdariffa solution is a cheap, natural, and harmless dye that can be used for staining of histological sections and it is comparable to the routine Hematoxylin and Eosin stain.

Watery extract of kujarat flowers need chemical purification to separate its acidic from basic components and this part will have closer characterization to the eosin stain.

Haematoxylin is an indispensable dye in histochemistry and histopathology and in cytology for the diagnosis of malignant and non malignant lesions and in immunohistochemistry.

These methods, which have been improved and extended over the years, are used for the examination of dyes and stains submitted to the BSC for certi®cation.